|
KMID : 1094720130180030486
|
|
Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 3 p.486 ~ p.490
|
|
|
Improvement of the steroid dihydroxylation efficiency from dehydroepiandrosterone using a substrate pre-induction biotransformation process
|
|
Li Hui
Fu Zhenzhen
Li Heng
Dou Wenfang
Shi Jinsong
Xu Zhenghong
|
|
|
Abstract
|
|
|
|
This study investigated the effects of hydroxylase cyptochrome P450 inducers on the efficiency of the biotransformation of dehydroepiandrosterone (DHEA) to 3β, 7α, 15α-trihydroxy-5-androsten-17-one (7α,15α-diOHDHEA) by Colletotrichum lini ST-1. Special attention was given to the substrate DHEA being the best inducer and the fact that it could improve the yield of 7α, 15α-diOHDHEA. Based on the effects of the DHEA pre-induction phases and additional concentrations on 7α, 15α-diOHDHEA production, a substrate pre-induction process was established as follows: 2 g/L DHEA was added for the first time after 12 h of inoculation, followed by the second addition of 6 g/L DHEA after 12 h later. The results showed that this substrate pre-induction process improved the content of cytochrome P450 and that the 7α, 15α-diOH-DHEA yield reached 90.1%, which was 26.9% higher than that obtained in the original process.
|
|
|
KEYWORD
|
|
|
DHEA, 7α, 15α-diOH-DHEA, Colletotrichum lini ST-1, dihydroxylation, pre-induction
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|
|